bright field transmission microscope  (Nikon)

Journal: iScience

Article Title: USP18 promotes clear cell renal cell carcinoma progression by regulating the ubiquitination and stability of YBX3

doi: 10.1016/j.isci.2026.115808

Figure Lengend Snippet: USP18 deficiency inhibits the growth of ccRCC organoids (A and B) Bright-field (A, scale bars, 500 μm) and H&E (B, scale bars, 100 μm) images showing the morphology and structure of ccRCC organoids from three patient samples. (C and D) IF data show the expression of CK7 (C) and Vimentin (D) in ccRCC organoids (scale bars, 100 μm). (E) Bright-field images showing the morphology of control and USP18-deficient ccRCC organoids. “Before” indicates the bright-field image of the organoids after transfection with shNC/shUSP18 at 72 h, and “after” indicates the bright-field image of the organoids after transfection with shNC/shUSP18 at 96 h. The images taken before and after are of the same organoids at different time points in the same field of view. (F) Change in diameter (%) of control and USP18-deficient ccRCC organoids. (G) ATP assay indicating the viability of control and USP18-deficient ccRCC organoids. (H and I) Western blot data indicate the expression of USP18, YBX3, P-AKT, and P-PI3K in control and USP18-deficient ccRCC organoids. Unpaired two-tailed Student’s t test was employed for p -value calculation. Data are represented as mean ± SD.

Article Snippet: Inverted bright-field microscope , Motic , N/A.

Techniques: Expressing, Control, Transfection, ATP Assay, Western Blot, Two Tailed Test

Journal: Chembiochem

Article Title: Droplet Microfluidics‐Assisted Fabrication of Magnetite Nanoparticle Hybrid Microgels for Facile Protein Immobilization

doi: 10.1002/cbic.202500958

Figure Lengend Snippet: Fabrication and stability of biotin‐functionalized hybrid microgels loaded with MNPs. (A) Scheme illustrating droplet microfluidics‐assisted fabrication of biotin‐functionalized, poly(acrylamide)‐based hybrid microgels with MNPs. HµGel‐XB denotes biotinylated microgel, where X indicates biotin‐PEG‐acrylamide concentration in the precursor solution in % ( w/w ). Following ultrasonic treatment, the precursor solution is injected into microfluidic channels with flow‐focusing design (as shown in the AutoCAD design). Crosslinking of water‐in‐oil (W/O) emulsion droplets occurs via UV irradiation within the outflow tubing. (B) MNP stability in the precursor solution over time demonstrated by bright‐field microscopy images of purified microgels containing 1% ( w/w ) MNP and 2.5% biotin (HµGel‐2.5B) after 1.5 and 3.5 h of experimentation, respectively. Insets show uniform distribution of MNPs within the microgels and reveal consistent MNP loading during microfluidic processing over time. All scale bars denote 150 μm.

Article Snippet: Droplet formation was monitored through a high‐speed camera (Miro C110, Vision Research Inc., Wayne, NJ, USA) coupled to an inverted bright‐field microscope (10× objective lens, air, Axio Vert.A1, Carl Zeiss, Oberkochen, Germany).

Techniques: Concentration Assay, Injection, Emulsion, Irradiation, Microscopy, Purification

Journal: Chembiochem

Article Title: Droplet Microfluidics‐Assisted Fabrication of Magnetite Nanoparticle Hybrid Microgels for Facile Protein Immobilization

doi: 10.1002/cbic.202500958

Figure Lengend Snippet: Immobilization of sfGFP‐Mad10trunc‐His fusion protein on MNPs embedded inside pAAm‐based hybrid microgels. (A) 10× and (B) 40× bright‐field and corresponding CLSM microscopy images showing the selective binding of sfGFP‐Mad10trunc‐His to MNPs within HµGel‐1.25B after 3 days of incubation. All scale bars denote 150 µm.

Article Snippet: Droplet formation was monitored through a high‐speed camera (Miro C110, Vision Research Inc., Wayne, NJ, USA) coupled to an inverted bright‐field microscope (10× objective lens, air, Axio Vert.A1, Carl Zeiss, Oberkochen, Germany).

Techniques: Microscopy, Binding Assay, Incubation

Journal: STAR Protocols

Article Title: Protocol to enhance pre-sexual and sexual differentiation of Toxoplasma gondii using retinal cells and intestinal organoid-derived monolayers

doi: 10.1016/j.xpro.2026.104367

Figure Lengend Snippet: Workflow for intestinal organoid generation and monolayer preparation (A) Schematic representation of tissue processing, crypt isolation, and embedding in BME domes to establish 3D intestinal organoids. (B) Overview of organoid dissociation and seeding onto wells to generate organoid-derived monolayers (ODMs). (C) BME domes containing organoids; (D) Bright field image of organoids ready for ODM seeding; (E) Bright field image of organoids not suitable for ODM seeding. They have overgrown and there are too many dead cells in the lumen. Scale bar: 100 μm. Created with Biorender.com.

Article Snippet: Bright-field inverted microscope (20× 0.3 NA and 40× 0.8 NA objectives) , Zeiss , Primovert.

Techniques: Isolation, Derivative Assay